TY - JOUR
T1 - Expeditious synthesis of a new hexasaccharide using transglycosylation reaction catalyzed by Bacillus (1→3),(1→4)-β-D-glucan 4-glucanohydrolase
AU - Viladot, Josep Lluís
AU - Stone, Bruce
AU - Driguez, Hugues
AU - Planas, Antoni
PY - 1998/9
Y1 - 1998/9
N2 - Enzymatic hydrolysis of barley (1→3),(1→4)-β-D-glucan using a recombinant (1→3),(1→4)-β-glucanase from Bacillus licheniformis gives Glcβ4Glcβ3Glc isolated after acetylation in 49% yield. Conventional treatment produced the corresponding β-fluoride which was carefully de-O-acetylated. A transglycosylation reaction with this substrate, catalyzed by the title enzyme, gave Glcβ4Glcβ3Glcβ4Glcβ4Glcβ3Glc in 20% yield. Copyright (C) 1997 Elsevier Science Ltd.
AB - Enzymatic hydrolysis of barley (1→3),(1→4)-β-D-glucan using a recombinant (1→3),(1→4)-β-glucanase from Bacillus licheniformis gives Glcβ4Glcβ3Glc isolated after acetylation in 49% yield. Conventional treatment produced the corresponding β-fluoride which was carefully de-O-acetylated. A transglycosylation reaction with this substrate, catalyzed by the title enzyme, gave Glcβ4Glcβ3Glcβ4Glcβ4Glcβ3Glc in 20% yield. Copyright (C) 1997 Elsevier Science Ltd.
KW - Enzymatic synthesis
KW - Glycosyl fluoride
KW - Hexasaccharide
KW - Transglycosylation; (1→3),(1→4)-β-glucanase
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U2 - 10.1016/S0008-6215(98)00212-2
DO - 10.1016/S0008-6215(98)00212-2
M3 - Article
C2 - 9821269
AN - SCOPUS:0031657592
SN - 0008-6215
VL - 311
SP - 95
EP - 99
JO - Carbohydrate Research
JF - Carbohydrate Research
IS - 1-2
ER -