Differentiation of Mouse Embryonic Stem Cells in Self-Assembling Peptide Scaffolds

Núria Marí-Buyé, Carlos E. Semino

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Here, we describe the capacity of mouse embryonic stem cells (mESCs) to differentiate into osteoblast-like cells in a three-dimensional (3D) self-assembling peptide scaffold, a synthetic nanofiber biomaterial with future applications in regenerative medicine. We have previously demonstrated that classical tissue cultures (two-dimensional) as well as 3D-systems promoted differentiation of mESCs into cells with an osteoblast-like phenotype expressing osteopontin (OPN) and collagen type I (Col I), as well as high alkaline phosphatase (Alk Phos) activity and calcium phosphate mineralization. Interestingly, in 3D self-assembling peptide scaffold cultures, the frequency of appearance of embryonic stem-cell-like colonies was substantially enhanced, suggesting that this particular 3D microenvironment promoted the generation of a stem-cell-like niche that allows the maintenance of a small pool of undifferentiated cells. We propose that the 3D system provides a unique microenvironment permissive to promote differentiation of mESCs into osteoblast-like cells while maintaining its regenerative capacity.

Idioma originalAnglès
Títol de la publicacióEmbryonic Stem Cell Therapy for Osteo-Degenerative Diseases
Subtítol de la publicacióMethods and Protocols
EditorHumana Press Inc.
Nombre de pàgines21
ISBN (imprès)9781607619611
Estat de la publicacióPublicada - 2011

Sèrie de publicacions

NomMethods in Molecular Biology
ISSN (imprès)1064-3745
ISSN (electrònic)1940-6029


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