TY - JOUR
T1 - Development of a Self-Assembled Peptide/Methylcellulose-Based Bioink for 3D Bioprinting
AU - Cofiño, Carla
AU - Perez-Amodio, Soledad
AU - Semino, Carlos E.
AU - Engel, Elisabeth
AU - Mateos-Timoneda, Miguel A.
N1 - Funding Information:
Part of this work was supported by the Spanish Ministry of Economy and Competitiveness through the project [MAT2015-68906-R] (MINECO/FEDER) and CERCA Programme/Generalitat de Catalunya. This work has been developed in the context of QuirofAM project (COMRDI16-1-0011) with the support of ACCIÓ (Catalonia Trade & Investment; Generalitat de Catalunya) and the European Community under the Catalonian ERDF operational program (European Regional Development Fund) 2014–2020. Part of the work has been performed at the ICTS “NANBIOSIS,” Unit 5 of CIBER in Bioengineering, Biomaterials & Nanomedicine (CIBER-BBN) at IBEC.
Funding Information:
Part of this work was supported by the Spanish Ministry of Economy and Competitiveness through the project [MAT2015-68906-R] (MINECO/ FEDER) and CERCA Programme/Generalitat de Catalunya. This work has been developed in the context of QuirofAM project (COMRDI16-1-0011) with the support of ACCIÓ (Catalonia Trade & Investment; Generalitat de Catalunya) and the European Community under the Catalonian ERDF operational program (European Regional Development Fund) 2014– 2020. Part of the work has been performed at the ICTS “NANBIOSIS,” Unit 5 of CIBER in Bioengineering, Biomaterials & Nanomedicine (CIBER-BBN) at IBEC.
Publisher Copyright:
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2019/11/1
Y1 - 2019/11/1
N2 - The introduction of 3D bioprinting to fabricate living constructs with tailored architecture has provided a new paradigm for biofabrication, with the potential to overcome several drawbacks of conventional scaffold-based tissue regeneration strategies. Hydrogel-based materials are suitable candidates regarding cell biocompatibility but often display poor mechanical properties. Self-assembling peptides are a promising source of biomaterials to be used as 3D scaffolds based on their similarity to extracellular matrices (structurally and mechanically). In this study, an advanced bioink for biofabrication is presented based on the optimization of a RAD16-I-based biomaterial. The strategy followed to build 3D predefined structures by 3D printing is based on an enhancement of bioink viscosity by adding methylcellulose (MC) to a RAD16-I solution. The resultant constructs display high shape fidelity and stability and embedded human mesenchymal stem cells present high viability after 7 days of culture. Moreover, cells are also able to differentiate to the adipogenic lineage, suggesting the suitability of this novel biomaterial for soft tissue engineering applications.
AB - The introduction of 3D bioprinting to fabricate living constructs with tailored architecture has provided a new paradigm for biofabrication, with the potential to overcome several drawbacks of conventional scaffold-based tissue regeneration strategies. Hydrogel-based materials are suitable candidates regarding cell biocompatibility but often display poor mechanical properties. Self-assembling peptides are a promising source of biomaterials to be used as 3D scaffolds based on their similarity to extracellular matrices (structurally and mechanically). In this study, an advanced bioink for biofabrication is presented based on the optimization of a RAD16-I-based biomaterial. The strategy followed to build 3D predefined structures by 3D printing is based on an enhancement of bioink viscosity by adding methylcellulose (MC) to a RAD16-I solution. The resultant constructs display high shape fidelity and stability and embedded human mesenchymal stem cells present high viability after 7 days of culture. Moreover, cells are also able to differentiate to the adipogenic lineage, suggesting the suitability of this novel biomaterial for soft tissue engineering applications.
KW - 3D bioprinting
KW - biofabrication
KW - bioinks
KW - self-assembling peptides
KW - tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=85073624078&partnerID=8YFLogxK
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=pure_univeritat_ramon_llull&SrcAuth=WosAPI&KeyUT=WOS:000479661000001&DestLinkType=FullRecord&DestApp=WOS_CPL
U2 - 10.1002/mame.201900353
DO - 10.1002/mame.201900353
M3 - Article
AN - SCOPUS:85073624078
SN - 1438-7492
VL - 304
JO - Macromolecular Materials and Engineering
JF - Macromolecular Materials and Engineering
IS - 11
M1 - 1900353
ER -