TY - JOUR
T1 - Detection of peptide-based nanoparticles in blood plasma by ELISA
AU - Bode, Gerard H.
AU - Pickl, Karin E.
AU - Sanchez-Purrà, Maria
AU - Albaiges, Berta
AU - Borrós, Salvador
AU - Pötgens, Andy J.G.
AU - Schmitz, Christoph
AU - Sinner, Frank M.
AU - Losen, Mario
AU - Steinbusch, Harry W.M.
AU - Frank, Hans Georg
AU - Martinez-Martinez, Pilar
AU - Wagemann, Kurt
AU - Klok, Harm Anton
AU - Unger, Ronald E.
AU - Pieber, Thomas
AU - Cesàro, Attilio
AU - Engbersen, Johan
AU - Kasemo, Bengt
AU - Moeller, Martin
AU - Korenstein, Rafi
AU - Grandfils, Christian
AU - Bernkop-Schnuerch, Andreas
AU - Kiparissides, Costas
AU - Slomkowski, Stanislaw
AU - Venturini, Peter
AU - Paleos, Constantinos M.
AU - Podobnik, Barbara
AU - Borm, Paul
AU - Van Winden, Ewoud C.A.
AU - Groll, Juergen
AU - Zassler, Birgit
AU - Gregoriadis, Gregory
AU - Nielsen, Peter Kresten
AU - Elouahabi, Abdelatif
N1 - Publisher Copyright:
© 2015 Bode et al.
PY - 2015/5/21
Y1 - 2015/5/21
N2 - Aims: The aim of the current study was to develop a method to detect peptide-linked nanoparticles in blood plasma. Materials & Methods: A convenient enzyme linked immunosorbent assay (ELISA) was developed for the detection of peptides functionalized with biotin and fluorescein groups. As a proof of principle, polymerized pentafluorophenyl methacrylate nanoparticles linked to biotin-carboxyfluorescein labeled peptides were intravenously injected in Wistar rats. Serial blood plasma samples were analyzed by ELISA and by liquid chromatography mass spectrometry (LC/MS) technology. Results: The ELISA based method for the detection of FITC labeled peptides had a detection limit of 1 ng/mL. We were able to accurately measure peptides bound to pentafluorophenyl meth-acrylate nanoparticles in blood plasma of rats, and similar results were obtained by LC/MS. Conclusions: We detected FITC-labeled peptides on pentafluorophenyl methacrylate nanoparticles after injection in vivo. This method can be extended to detect nanoparticles with different chemical compositions.
AB - Aims: The aim of the current study was to develop a method to detect peptide-linked nanoparticles in blood plasma. Materials & Methods: A convenient enzyme linked immunosorbent assay (ELISA) was developed for the detection of peptides functionalized with biotin and fluorescein groups. As a proof of principle, polymerized pentafluorophenyl methacrylate nanoparticles linked to biotin-carboxyfluorescein labeled peptides were intravenously injected in Wistar rats. Serial blood plasma samples were analyzed by ELISA and by liquid chromatography mass spectrometry (LC/MS) technology. Results: The ELISA based method for the detection of FITC labeled peptides had a detection limit of 1 ng/mL. We were able to accurately measure peptides bound to pentafluorophenyl meth-acrylate nanoparticles in blood plasma of rats, and similar results were obtained by LC/MS. Conclusions: We detected FITC-labeled peptides on pentafluorophenyl methacrylate nanoparticles after injection in vivo. This method can be extended to detect nanoparticles with different chemical compositions.
KW - Drug-delivery
KW - Nanomedicine
KW - Atherosclerosis
KW - Cancer
KW - Nap
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U2 - 10.1371/journal.pone.0126136
DO - 10.1371/journal.pone.0126136
M3 - Article
C2 - 25996618
AN - SCOPUS:84930669322
SN - 1932-6203
VL - 10
JO - PLoS ONE
JF - PLoS ONE
IS - 5
M1 - e0126136
ER -
