Concanavalin A Delivers a Photoactive Protein to the Bacterial Wall

Andrea Mussini, Pietro Delcanale, Melissa Berni, Stefano Pongolini, Mireia Jordà-Redondo, Montserrat Agut, Peter J. Steinbach, Santi Nonell, Stefania Abbruzzetti, Cristiano Viappiani

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Resum

Modular supramolecular complexes, where different proteins are assembled to gather targeting capability and photofunctional properties within the same structures, are of special interest for bacterial photodynamic inactivation, given their inherent biocompatibility and flexibility. We have recently proposed one such structure, exploiting the tetrameric bacterial protein streptavidin as the main building block, to target S. aureus protein A. To expand the palette of targets, we have linked biotinylated Concanavalin A, a sugar-binding protein, to a methylene blue-labelled streptavidin. By applying a combination of spectroscopy and microscopy, we demonstrate the binding of Concanavalin A to the walls of Gram-positive S. aureus and Gram-negative E. coli. Photoinactivation is observed for both bacterial strains in the low micromolar range, although the moderate affinity for the molecular targets and the low singlet oxygen yields limit the overall efficiency. Finally, we apply a maximum entropy method to the analysis of autocorrelation traces, which proves particularly useful when interpreting signals measured for diffusing systems heterogeneous in size, such as fluorescent species bound to bacteria.

Idioma originalAnglès
Número d’article5751
RevistaInternational Journal of Molecular Sciences
Volum25
Número11
DOIs
Estat de la publicacióPublicada - de juny 2024

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