TY - JOUR
T1 - C20-nor-Abietane and Three Abietane Diterpenoids from Plectranthus mutabilis Leaves as P-Glycoprotein Modulators
AU - Ntungwe, Epole N.
AU - Stojanov, Sofija Jovanovic
AU - Duarte, Noelia M.
AU - Candeias, Nuno R.
AU - Diaz-Lanza, Ana M.
AU - Vagvolgyi, Mate
AU - Hunyadi, Attila
AU - Pesic, Milica
AU - Rijo, Patricia
PY - 2022/4/14
Y1 - 2022/4/14
N2 - In this study, a bioguided fractionation ofPlectranthus mutabilisextract was performed by chromatographicmethods. It yielded one newnor-abietane diterpene, mutabilol (1),and three known abietanes, coleon-U-quinone (2), 8 alpha,9 alpha-epoxycoleon-U-quinone (3), and coleon U (4). The abietanediterpenoid5was also tentatively identified using HPLC-MS/MS.Moreover, the extract profile and quantification of each isolatedcompound were determined by HPLC-DAD. Compound4was themajor compound in the extract. Compounds2-4were found to beselective toward cancer cell lines and were able to inhibit P-glycoprotein (P-gp) activity in NCI-H460/R cells at longerexposure of 72 h and consequently revert doxorubicin (DOX)resistance in subsequent combined treatment. None of thecompounds influenced the P-gp expression in NCI-H460/R cells, while the extract significantly increased it
AB - In this study, a bioguided fractionation ofPlectranthus mutabilisextract was performed by chromatographicmethods. It yielded one newnor-abietane diterpene, mutabilol (1),and three known abietanes, coleon-U-quinone (2), 8 alpha,9 alpha-epoxycoleon-U-quinone (3), and coleon U (4). The abietanediterpenoid5was also tentatively identified using HPLC-MS/MS.Moreover, the extract profile and quantification of each isolatedcompound were determined by HPLC-DAD. Compound4was themajor compound in the extract. Compounds2-4were found to beselective toward cancer cell lines and were able to inhibit P-glycoprotein (P-gp) activity in NCI-H460/R cells at longerexposure of 72 h and consequently revert doxorubicin (DOX)resistance in subsequent combined treatment. None of thecompounds influenced the P-gp expression in NCI-H460/R cells, while the extract significantly increased it
KW - P-glycoprotei
KW - Plectranthus mutabilis
KW - Abietane diterpenoids
KW - Anticancer effect
KW - Multidrug resistance
UR - http://www.scopus.com/inward/record.url?scp=85126589451&partnerID=8YFLogxK
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=pure_univeritat_ramon_llull&SrcAuth=WosAPI&KeyUT=WOS:000791541600029&DestLinkType=FullRecord&DestApp=WOS_CPL
U2 - 10.1021/acsmedchemlett.1c00711
DO - 10.1021/acsmedchemlett.1c00711
M3 - Article
C2 - 35450348
SN - 1948-5875
VL - 13
SP - 674
EP - 680
JO - Acs Medicinal Chemistry Letters
JF - Acs Medicinal Chemistry Letters
IS - 4
ER -
