TY - JOUR
T1 - Bacterial 1,3-1,4-β-glucanases
T2 - Structure, function and protein engineering
AU - Planas, A.
N1 - Funding Information:
The author thanks Drs R. Borriss (Humboldt Universität, Berlin), U. Christensen (University of Copenhagen), H. Driguez (CERMAV, CNRS, Grenoble), U. Heinemann (Max-Delbrück Centrum, Berlin) and E. Querol (Universitat Autònoma de Barcelona) for collaborative work on 1,3-1,4-β-glucanases. Work from the author’s laboratory referred in this review was supported by Grants BIO94-0912-C02 and BIO97-0511-C02 from the Ministerio de Educación y Ciencia, Spain.
PY - 2000/12/29
Y1 - 2000/12/29
N2 - 1,3-1,4-β-Glucanases (or lichenases, EC 3.2.1.73) hydrolyse linear β-glucans containing β-1,3 and β-1,4 linkages such as cereal β-glucans and lichenan, with a strict cleavage specificity for β-1,4 glycosidic bonds on 3-O-substituted glucosyl residues. The bacterial enzymes are retaining glycosyl hydrolases of family 16 with a jellyroll β-sandwich fold and a substrate binding cleft composed of six subsites. The present paper reviews the structure-function aspects of the enzymatic action including mechanistic enzymology, protein engineering and X-ray crystallographic studies.
AB - 1,3-1,4-β-Glucanases (or lichenases, EC 3.2.1.73) hydrolyse linear β-glucans containing β-1,3 and β-1,4 linkages such as cereal β-glucans and lichenan, with a strict cleavage specificity for β-1,4 glycosidic bonds on 3-O-substituted glucosyl residues. The bacterial enzymes are retaining glycosyl hydrolases of family 16 with a jellyroll β-sandwich fold and a substrate binding cleft composed of six subsites. The present paper reviews the structure-function aspects of the enzymatic action including mechanistic enzymology, protein engineering and X-ray crystallographic studies.
KW - 1,3-1,4-β-glucanases
KW - Mechanism
KW - Protein engineering
KW - Specificity
KW - Structure
UR - http://www.scopus.com/inward/record.url?scp=0034731485&partnerID=8YFLogxK
U2 - 10.1016/S0167-4838(00)00231-4
DO - 10.1016/S0167-4838(00)00231-4
M3 - Review
C2 - 11150614
AN - SCOPUS:0034731485
SN - 0167-4838
VL - 1543
SP - 361
EP - 382
JO - Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology
JF - Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology
IS - 2
ER -