TY - JOUR
T1 - Assessment of chlorpyrifos toxic effects in zebrafish (Danio rerio) metabolism
AU - Gómez-Canela, Cristian
AU - Prats, Eva
AU - Piña, Benjamí
AU - Tauler, Romà
N1 - Funding Information:
The research leading to these results has received funding from the European Research Council under European Union's Seven Framework Programme (FP/2007–2013)/ERC Grant Agreement n. 320737 .
Publisher Copyright:
© 2016 Elsevier Ltd
PY - 2017/1/1
Y1 - 2017/1/1
N2 - In this work the effect of chlorpyrifos exposure on metabolic profiles of zebrafish muscle was evaluated by liquid chromatography coupled to high resolution mass spectrometry. Different chemometric tools based on the selection of Regions of Interest and on Multivariate Curve-Resolution-Alternating Least Squares are proposed for the analysis of the complex data sets generated in the different exposure experiments. Analysis of Variance Simultaneous Component Analysis of changes on metabolite peak profile areas showed significant chlorpyrifos concentration and exposure time-dependent changes, clearly differentiating between exposed and non-exposed samples and between short (2 h) and long exposure times (6 h or 24 h). The changes observed in the concentrations of 50 muscle metabolites are indicative of induction of oxidative stress, of a general disruption of neurotransmitter metabolism, and of muscle exhaustion. These three effects are intimately related to the toxicity of chlorpyrifos.
AB - In this work the effect of chlorpyrifos exposure on metabolic profiles of zebrafish muscle was evaluated by liquid chromatography coupled to high resolution mass spectrometry. Different chemometric tools based on the selection of Regions of Interest and on Multivariate Curve-Resolution-Alternating Least Squares are proposed for the analysis of the complex data sets generated in the different exposure experiments. Analysis of Variance Simultaneous Component Analysis of changes on metabolite peak profile areas showed significant chlorpyrifos concentration and exposure time-dependent changes, clearly differentiating between exposed and non-exposed samples and between short (2 h) and long exposure times (6 h or 24 h). The changes observed in the concentrations of 50 muscle metabolites are indicative of induction of oxidative stress, of a general disruption of neurotransmitter metabolism, and of muscle exhaustion. These three effects are intimately related to the toxicity of chlorpyrifos.
KW - Environmental pollution
KW - LC-HRMS
KW - Metabolomics
KW - ROI-MCR-ALS
KW - Zebrafish
UR - https://www.scopus.com/pages/publications/84999836238
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=pure_univeritat_ramon_llull&SrcAuth=WosAPI&KeyUT=WOS:000390732300051&DestLinkType=FullRecord&DestApp=WOS_CPL
U2 - 10.1016/j.envpol.2016.11.010
DO - 10.1016/j.envpol.2016.11.010
M3 - Article
C2 - 27839997
AN - SCOPUS:84999836238
SN - 0269-7491
VL - 220
SP - 1231
EP - 1243
JO - Environmental Pollution
JF - Environmental Pollution
ER -
