Assessing the potential of photosensitizing flavoproteins as tags for correlative microscopy

Alberto Rodríguez-Pulido; Aitziber L. Cortajarena; Joaquim Torra; Rubén Ruiz-González; Santi Nonell; Cristina Flors

doi:10.1039/c6cc03119f

Assessing the potential of photosensitizing flavoproteins as tags for correlative microscopy

Alberto Rodríguez-Pulido
, Aitziber L. Cortajarena
, Joaquim Torra
, Rubén Ruiz-González
, Santi Nonell
, Cristina Flors^*

^*Autor corresponent d’aquest treball

IQS School of Engineering

Producció científica: Article en revista indexada › Article › Avaluat per experts

33 Cites (Scopus)

Resum

Photosensitizing flavoproteins have great potential as tags for correlative light and electron microscopy (CLEM). We examine the photostability of miniSOG mutants and their ability to photo-oxidize diaminobenzidine, both key aspects for CLEM. Our experiments reveal a complex relation between these parameters and the production of different reactive oxygen species.

Idioma original	Anglès
Pàgines (de-a)	8405-8408
Nombre de pàgines	4
Revista	Chemical Communications
Volum	52
Número	54
DOIs	https://doi.org/10.1039/c6cc03119f
Estat de la publicació	Publicada - 11 de jul. 2016

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abstract = "Photosensitizing flavoproteins have great potential as tags for correlative light and electron microscopy (CLEM). We examine the photostability of miniSOG mutants and their ability to photo-oxidize diaminobenzidine, both key aspects for CLEM. Our experiments reveal a complex relation between these parameters and the production of different reactive oxygen species.",

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AU - Rodríguez-Pulido, Alberto

AU - Cortajarena, Aitziber L.

AU - Torra, Joaquim

AU - Ruiz-González, Rubén

AU - Nonell, Santi

AU - Flors, Cristina

PY - 2016/7/11

Y1 - 2016/7/11

N2 - Photosensitizing flavoproteins have great potential as tags for correlative light and electron microscopy (CLEM). We examine the photostability of miniSOG mutants and their ability to photo-oxidize diaminobenzidine, both key aspects for CLEM. Our experiments reveal a complex relation between these parameters and the production of different reactive oxygen species.

AB - Photosensitizing flavoproteins have great potential as tags for correlative light and electron microscopy (CLEM). We examine the photostability of miniSOG mutants and their ability to photo-oxidize diaminobenzidine, both key aspects for CLEM. Our experiments reveal a complex relation between these parameters and the production of different reactive oxygen species.

KW - Singlet oxygen photosensitization

KW - Fluorescent protein ilov

KW - Electron-microscopy

KW - Light

KW - Photooxidation

KW - Cytochemistry

KW - Photophysics

KW - Technology

KW - Generation

KW - Probes

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JO - Chemical Communications

JF - Chemical Communications

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Assessing the potential of photosensitizing flavoproteins as tags for correlative microscopy

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