Analysis of Ochratoxin A in Coffee by Solid-Phase Cleanup and Narrow-Bore Liquid Chromatography-Fluorescence Detector-Mass Spectrometry

Meritxell Ventura; Carlos Vallejos; Iván Atilio Anaya; Francesc Broto-Puig; Montserrat Agut; Lluís Comellas

doi:10.1021/jf034856+

Analysis of Ochratoxin A in Coffee by Solid-Phase Cleanup and Narrow-Bore Liquid Chromatography-Fluorescence Detector-Mass Spectrometry

Meritxell Ventura, Carlos Vallejos, Iván Atilio Anaya, Francesc Broto-Puig, Montserrat Agut, Lluís Comellas

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A method for the analysis of ochratoxin A (OTA) in green and roasted coffee has been developed. OTA was extracted from coffee with 1% NaHCO ₃, and the extract was filtered and purified by solid-phase cleanup using a polymeric column that exhibits reversed-phase and anion-exchange functionalities. OTA was analyzed on a narrow-bore reversed-phase C ₁₈ HPLC column with acetonitrile/water (0.1% formic acid) (40:60) as mobile phase and quantified with a fluorescence detector. The presence of OTA in coffee was confirmed by single-quadruple mass spectrometry using an electrospray ionization source. The method has been validated, obtaining a recovery of 82.5% and a detection limit of 0.1 ng/g. It has been applied to 20 coffee samples from various countries and different manufacturers with no detection of OTA.

Idioma original	Anglès
Pàgines (de-a)	7564-7567
Nombre de pàgines	4
Revista	Journal of Agricultural and Food Chemistry
Volum	51
Número	26
DOIs	https://doi.org/10.1021/jf034856+
Estat de la publicació	Publicada - 17 de des. 2003

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@article{74b8317236ab4818ac837367f530df49,

title = "Analysis of Ochratoxin A in Coffee by Solid-Phase Cleanup and Narrow-Bore Liquid Chromatography-Fluorescence Detector-Mass Spectrometry",

abstract = "A method for the analysis of ochratoxin A (OTA) in green and roasted coffee has been developed. OTA was extracted from coffee with 1% NaHCO 3, and the extract was filtered and purified by solid-phase cleanup using a polymeric column that exhibits reversed-phase and anion-exchange functionalities. OTA was analyzed on a narrow-bore reversed-phase C 18 HPLC column with acetonitrile/water (0.1% formic acid) (40:60) as mobile phase and quantified with a fluorescence detector. The presence of OTA in coffee was confirmed by single-quadruple mass spectrometry using an electrospray ionization source. The method has been validated, obtaining a recovery of 82.5% and a detection limit of 0.1 ng/g. It has been applied to 20 coffee samples from various countries and different manufacturers with no detection of OTA.",

keywords = "Coffee, HPLC, MS, Ochratoxin A, SPE",

author = "Meritxell Ventura and Carlos Vallejos and Anaya, {Iv{\'a}n Atilio} and Francesc Broto-Puig and Montserrat Agut and Llu{\'i}s Comellas",

year = "2003",

month = dec,

day = "17",

doi = "10.1021/jf034856+",

language = "English",

volume = "51",

pages = "7564--7567",

journal = "Journal of Agricultural and Food Chemistry",

issn = "0021-8561",

publisher = "American Chemical Society",

number = "26",

}

Analysis of Ochratoxin A in Coffee by Solid-Phase Cleanup and Narrow-Bore Liquid Chromatography-Fluorescence Detector-Mass Spectrometry. / Ventura, Meritxell; Vallejos, Carlos; Anaya, Iván Atilio et al.
In: Journal of Agricultural and Food Chemistry, Vol. 51, Núm. 26, 17.12.2003, pàg. 7564-7567.

Producció científica: Article en revista indexada › Article › Avaluat per experts

TY - JOUR

T1 - Analysis of Ochratoxin A in Coffee by Solid-Phase Cleanup and Narrow-Bore Liquid Chromatography-Fluorescence Detector-Mass Spectrometry

AU - Ventura, Meritxell

AU - Vallejos, Carlos

AU - Anaya, Iván Atilio

AU - Broto-Puig, Francesc

AU - Agut, Montserrat

AU - Comellas, Lluís

PY - 2003/12/17

Y1 - 2003/12/17

N2 - A method for the analysis of ochratoxin A (OTA) in green and roasted coffee has been developed. OTA was extracted from coffee with 1% NaHCO 3, and the extract was filtered and purified by solid-phase cleanup using a polymeric column that exhibits reversed-phase and anion-exchange functionalities. OTA was analyzed on a narrow-bore reversed-phase C 18 HPLC column with acetonitrile/water (0.1% formic acid) (40:60) as mobile phase and quantified with a fluorescence detector. The presence of OTA in coffee was confirmed by single-quadruple mass spectrometry using an electrospray ionization source. The method has been validated, obtaining a recovery of 82.5% and a detection limit of 0.1 ng/g. It has been applied to 20 coffee samples from various countries and different manufacturers with no detection of OTA.

AB - A method for the analysis of ochratoxin A (OTA) in green and roasted coffee has been developed. OTA was extracted from coffee with 1% NaHCO 3, and the extract was filtered and purified by solid-phase cleanup using a polymeric column that exhibits reversed-phase and anion-exchange functionalities. OTA was analyzed on a narrow-bore reversed-phase C 18 HPLC column with acetonitrile/water (0.1% formic acid) (40:60) as mobile phase and quantified with a fluorescence detector. The presence of OTA in coffee was confirmed by single-quadruple mass spectrometry using an electrospray ionization source. The method has been validated, obtaining a recovery of 82.5% and a detection limit of 0.1 ng/g. It has been applied to 20 coffee samples from various countries and different manufacturers with no detection of OTA.

KW - Coffee

KW - HPLC

KW - MS

KW - Ochratoxin A

KW - SPE

UR - http://www.scopus.com/inward/record.url?scp=0347480147&partnerID=8YFLogxK

U2 - 10.1021/jf034856+

DO - 10.1021/jf034856+

M3 - Article

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AN - SCOPUS:0347480147

SN - 0021-8561

VL - 51

SP - 7564

EP - 7567

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 26

ER -

Analysis of Ochratoxin A in Coffee by Solid-Phase Cleanup and Narrow-Bore Liquid Chromatography-Fluorescence Detector-Mass Spectrometry

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