An engineered E.coli strain for the production of glycoglycerolipids

Neus Mora-Buyé, Magda Faijes, Antoni Planas

Producció científica: Article en revista indexadaArticleAvaluat per experts

2 Cites (Scopus)

Resum

The glycolipid synthase MG517 from . Mycoplasma genitalium catalyzes the glucosyl transfer from UDPGlc to diacylglycerol producing glycoglycerolipids (GGL) (. Andrés et al., 2011). The enzyme was functional in . E. coli accumulating GGL in the plasma membrane. A metabolic engineering strategy for GGL production was evaluated using this microorganism. To increase the levels of GGL precursors, UDPGlc and diacylglycerol, GalU and PlsC enzymes involved in their biosynthesis were overexpressed. Seven engineered strains were obtained containing different combinations of the . mg517 with . galU and . plsC genes. Diacylglycerol synthesis showed to be limiting and the strain overexpressing MG517 and PlsC achieved the highest GGL yield. The new lipids were mono, di- and triglucosyldiacylglycerol with different acyl combinations in each compound. It indicates that the successive glucosyl transferase activities of MG517 have different acyl chain specificity for the acceptor substrate. GGL represented up to 6. mg per g of dry weight.

Idioma originalAnglès
Pàgines (de-a)551-559
Nombre de pàgines9
RevistaMetabolic Engineering
Volum14
Número5
DOIs
Estat de la publicacióPublicada - de set. 2012

Fingerprint

Navegar pels temes de recerca de 'An engineered E.coli strain for the production of glycoglycerolipids'. Junts formen un fingerprint únic.

Com citar-ho