TY - JOUR
T1 - Amino acid and amino sugar determination by derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate followed by high-performance liquid chromatography and fluorescence detection
AU - Díaz, J.
AU - Lliberia, J. Ll
AU - Comellas, L.
AU - Broto-Puig, F.
N1 - Funding Information:
The authors thank the CICYT (Spain) (Reference: NAT 91-0340), for financial support and J.LI.L1 gratefully acknowledges the support of DGICYT (Spain).
PY - 1996/1/5
Y1 - 1996/1/5
N2 - A method for the derivatization of amino acids with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) for use with HPLC was tested. The reproducibility, linearity and limits of detection are similar to those obtained with other methods, such as the o-phthalaldehyde and 9-fluorenylmethoxycarbonyl methods. The ease of sample preparation and the possibility of amino sugar derivatization and resolution are the reasons why this method was chosen for simultaneous amino acid and amino sugar analysis. The need for a previous step of amino sugar reduction was studied and it was concluded that it is not necessary.
AB - A method for the derivatization of amino acids with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) for use with HPLC was tested. The reproducibility, linearity and limits of detection are similar to those obtained with other methods, such as the o-phthalaldehyde and 9-fluorenylmethoxycarbonyl methods. The ease of sample preparation and the possibility of amino sugar derivatization and resolution are the reasons why this method was chosen for simultaneous amino acid and amino sugar analysis. The need for a previous step of amino sugar reduction was studied and it was concluded that it is not necessary.
UR - http://www.scopus.com/inward/record.url?scp=0030028276&partnerID=8YFLogxK
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=pure_univeritat_ramon_llull&SrcAuth=WosAPI&KeyUT=WOS:A1996TQ03700020&DestLinkType=FullRecord&DestApp=WOS_CPL
U2 - 10.1016/0021-9673(95)00372-X
DO - 10.1016/0021-9673(95)00372-X
M3 - Article
AN - SCOPUS:0030028276
SN - 0021-9673
VL - 719
SP - 171
EP - 179
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1
T2 - 23rd Scientific Meeting of the Group-of-Chromatography-and-Related-Techniques of the Spanish-Royal-Society-of-Chemistry
Y2 - 19 October 1994 through 21 October 1994
ER -