TY - JOUR
T1 - Activatable iodinated BODIPYs for selective imaging and photodynamic disruption of amyloid structures in pathogenic bacterial biofilms
AU - Torra, Joaquim
AU - Sawazaki, Taka
AU - Bondia, Patricia
AU - Nonell, Santi
AU - Kanai, Motomu
AU - Sohma, Youhei
AU - Flors, Cristina
N1 - Funding Information:
This research was supported by the Spanish Ministerio de Ciencia, Innovación y Universidades with projects PCI2018-093064 and PGC2018-094802-B-I00 (C.F.), SEV-2016-0686 (IMDEA Nanociencia) and by the Comunidad Autónoma de Madrid with project RENIM-CM S2017/BMD-3867 co-financed by European Structural and Investment Fund (C.F.). We also acknowledge the Japanese Agency for Medical Research and Development (AMED) for grants JP21jm0210058 (Y.S.) and JSPS KAKENHI JP20H00489 (M.K.), JP21H02602 (Y.S.), and JP21K20727 (T.S.). We thank the groups of Manuel Carmona (Centro de Investigaciones Biológicas-CSIC) and Luis Ángel Fernández (Centro Nacional de Biotecntología-CSIC) for kindly providing E. coli strains MC4100 and UTI89.
Funding Information:
This research was supported by the Spanish Ministerio de Ciencia, Innovación y Universidades with projects PCI2018-093064 and PGC2018-094802-B-I00 (C.F.), SEV-2016-0686 ( IMDEA Nanociencia ) and by the Comunidad Autónoma de Madrid with project RENIM-CM S2017/BMD-3867 co-financed by European Structural and Investment Fund (C.F.). We also acknowledge the Japanese Agency for Medical Research and Development (AMED) for grants JP21jm0210058 (Y.S.) and JSPS KAKENHI JP20H00489 (M.K.), JP21H02602 (Y.S.), and JP21K20727 (T.S.). We thank the groups of Manuel Carmona (Centro de Investigaciones Biológicas-CSIC) and Luis Ángel Fernández (Centro Nacional de Biotecntología-CSIC) for kindly providing E. coli strains MC4100 and UTI89.
Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/11/15
Y1 - 2022/11/15
N2 - We report the application of two iodinated boron dipyrromethene (BODIPY) photocatalysts as theranostic compounds against pathogenic bacterial biofilms. These derivatives, which are activated through a polarity effect upon binding, have exquisite selectivity towards amyloids in the biofilm extracellular matrix (ECM). This property can be exploited in fluorescence microscopy to stain the ECM of uropathogenic bacterial biofilms, displaying a superior selectivity compared to Thioflavin T, the gold standard for amyloid detection. Moreover, we show that the ability of these compounds to photosensitize reactive oxygen species upon binding can be used to weaken the mechanical barrier of the ECM and restore the activity of conventional antimicrobial agents, whose effect is strongly limited in intact biofilms. The combination of activatable near-infrared fluorescence and photo-oxygenation properties of these compounds upon binding to amyloid structures provides a powerful theranostic tool for simultaneous detection and treatment of pathogenic biofilm infections.
AB - We report the application of two iodinated boron dipyrromethene (BODIPY) photocatalysts as theranostic compounds against pathogenic bacterial biofilms. These derivatives, which are activated through a polarity effect upon binding, have exquisite selectivity towards amyloids in the biofilm extracellular matrix (ECM). This property can be exploited in fluorescence microscopy to stain the ECM of uropathogenic bacterial biofilms, displaying a superior selectivity compared to Thioflavin T, the gold standard for amyloid detection. Moreover, we show that the ability of these compounds to photosensitize reactive oxygen species upon binding can be used to weaken the mechanical barrier of the ECM and restore the activity of conventional antimicrobial agents, whose effect is strongly limited in intact biofilms. The combination of activatable near-infrared fluorescence and photo-oxygenation properties of these compounds upon binding to amyloid structures provides a powerful theranostic tool for simultaneous detection and treatment of pathogenic biofilm infections.
KW - Amyloid
KW - Bacterial biofilms, fluorescent probes, photodynamic therapy
KW - Theranostics
UR - http://www.scopus.com/inward/record.url?scp=85135890444&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2022.132475
DO - 10.1016/j.snb.2022.132475
M3 - Article
AN - SCOPUS:85135890444
SN - 0925-4005
VL - 371
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
M1 - 132475
ER -